express scribe transcription software version 6.10 Search Results


express scribe transcription software version 6.10  (NCH Software)
90
NCH Software express scribe transcription software version 6.10
Express Scribe Transcription Software Version 6.10, supplied by NCH Software, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/express+scribe+transcription+software+version+6%2E10/pm38907014-43-5-11?v=NCH+Software
Average 90 stars, based on 1 article reviews
express scribe transcription software version 6.10 - by Bioz Stars, 2026-08
90/100 stars
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ist1 rabbit polyclonal proteintech  (Proteintech)
96
Proteintech ist1 rabbit polyclonal proteintech
FIGURE 1 <t>IST1</t> functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.
Ist1 Rabbit Polyclonal Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/express+scribe+transcription+software+version+6%2E10/pm37926552-239-33-36?v=Proteintech
Average 96 stars, based on 1 article reviews
ist1 rabbit polyclonal proteintech - by Bioz Stars, 2026-08
96/100 stars
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express scribe transcription software  (NCH Software)
90
NCH Software express scribe transcription software
FIGURE 1 <t>IST1</t> functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.
Express Scribe Transcription Software, supplied by NCH Software, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/express+scribe+transcription+software+version+6%2E10/ppr0815130-39-5-11?v=NCH+Software
Average 90 stars, based on 1 article reviews
express scribe transcription software - by Bioz Stars, 2026-08
90/100 stars
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il 6  (R&D Systems)
94
R&D Systems il 6
FIGURE 1 <t>IST1</t> functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.
Il 6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/express+scribe+transcription+software+version+6%2E10/pm37235663-212-19-23?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
il 6 - by Bioz Stars, 2026-08
94/100 stars
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hle extended leads  (NANOBASE Inc)
90
NANOBASE Inc hle extended leads
FIGURE 1 <t>IST1</t> functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.
Hle Extended Leads, supplied by NANOBASE Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/express+scribe+transcription+software+version+6%2E10/us11248055-737-2-5?v=NANOBASE+Inc
Average 90 stars, based on 1 article reviews
hle extended leads - by Bioz Stars, 2026-08
90/100 stars
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maltron bf900  (Maltron International Ltd)
90
Maltron International Ltd maltron bf900
FIGURE 1 <t>IST1</t> functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.
Maltron Bf900, supplied by Maltron International Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/express+scribe+transcription+software+version+6%2E10/pmc11743147__41746_2024_1380_MOESM1_ESM-28-104-104?v=Maltron+International+Ltd
Average 90 stars, based on 1 article reviews
maltron bf900 - by Bioz Stars, 2026-08
90/100 stars
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marvin 6.1.0  (ChemAxon LLC)
90
ChemAxon LLC marvin 6.1.0
FIGURE 1 <t>IST1</t> functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.
Marvin 6.1.0, supplied by ChemAxon LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/express+scribe+transcription+software+version+6%2E10/10__1021_slash_acs__jmedchem__8b01529-627-0-2?v=ChemAxon+LLC
Average 90 stars, based on 1 article reviews
marvin 6.1.0 - by Bioz Stars, 2026-08
90/100 stars
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xmm-newton analysis survey (sas) v.6.1.0  (SAS institute)
90
SAS institute xmm-newton analysis survey (sas) v.6.1.0
FIGURE 1 <t>IST1</t> functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.
Xmm Newton Analysis Survey (Sas) V.6.1.0, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/express+scribe+transcription+software+version+6%2E10/10__1051_slash_0004___6361_ascii58_20054562-62-12-11?v=SAS+institute
Average 90 stars, based on 1 article reviews
xmm-newton analysis survey (sas) v.6.1.0 - by Bioz Stars, 2026-08
90/100 stars
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software netzsch proteus version 6.1.0  (NETZSCH)
90
NETZSCH software netzsch proteus version 6.1.0
FIGURE 1 <t>IST1</t> functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.
Software Netzsch Proteus Version 6.1.0, supplied by NETZSCH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/express+scribe+transcription+software+version+6%2E10/10__1002_slash_app__50730-57-3-2?v=NETZSCH
Average 90 stars, based on 1 article reviews
software netzsch proteus version 6.1.0 - by Bioz Stars, 2026-08
90/100 stars
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echopac pc 6.1.0  (Vingmed AS)
90
Vingmed AS echopac pc 6.1.0
FIGURE 1 <t>IST1</t> functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.
Echopac Pc 6.1.0, supplied by Vingmed AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/express+scribe+transcription+software+version+6%2E10/pmc05391073-93-25-30?v=Vingmed+AS
Average 90 stars, based on 1 article reviews
echopac pc 6.1.0 - by Bioz Stars, 2026-08
90/100 stars
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datlab 6 1 0 7  (Oroboros Instruments)
86
Oroboros Instruments datlab 6 1 0 7
FIGURE 1 <t>IST1</t> functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.
Datlab 6 1 0 7, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
datlab 6 1 0 7 - by Bioz Stars, 2026-08
86/100 stars
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graphpad/prism software  (GraphPad Software Inc)
90
GraphPad Software Inc graphpad/prism software
FIGURE 1 <t>IST1</t> functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.
Graphpad/Prism Software, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/express+scribe+transcription+software+version+6%2E10/pmc08524195__mmc1-8-99-5?v=GraphPad+Software+Inc
Average 90 stars, based on 1 article reviews
graphpad/prism software - by Bioz Stars, 2026-08
90/100 stars
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FIGURE 1 IST1 functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.

Journal: Traffic (Copenhagen, Denmark)

Article Title: IST1 regulates select recycling pathways.

doi: 10.1111/tra.12921

Figure Lengend Snippet: FIGURE 1 IST1 functions on early/sorting endosomes in tubule scission (A) U2OS cell stained for endogenous IST1, asterisk denotes IST1 at cytokinetic cell bridges and mid-bodies. (B) U2OS (right) and T24 (left) cells immunostained with IST1 and EEA1. (C) Immunostaining of EEA1 in control and IST1 siRNA treated T24 cells, and the recruitment of EEA1 is quantitated to the right, n = 17 for control, n = 19 for IST1siRNA treated. The graph shows median ± 95% CI. Statistical analysis with Mann–Whitney U test showed a significant increase in EEA1 in IST1-depleted cells. These results were observed for 3 independent experiments. Images in (A–C) are max intensity projections. (D–F) Stable U2OS cell lines expressing GFP-Rab5 were treated with IST1, CHMP1B or the unrelated protein LAP1 (control) siRNA, and Rab5 tubule number and duration were assessed by live cell imaging. (D) Representative single frames, single slices from live cell movies. Note the presence of long tubules (inset) in cells depleted of CHMP1B and IST1. (E) Each dot in the scatter plots shows the number of tubules in a single cell longer than 5 μm and present for >2 s over a 30 s recording, n = 50 for all three groups (results are pooled from three independent experiments). The graph shows median ± 95% CI. Statistical analysis using Kruskal–Wallis test revealed an increased number of tubules per cell for IST1 and CHMP1B- depleted cells. (F) Bar graph totals tubules counted in (E) and further shows how long each was present (tubule duration). Not only did CHMP1B and IST1 KD cells have more tubules, but only five tubules observed in control KD cells persisted for over 4 s while 43 and 14 tubules observed in CHMP1B and IST1 KD cells, respectively, persisted for over 4 s. Scale bars in the whole cells and insets are as follows: (A) 25 μm, (B) 25 and 1 μm, (C) 25 μm and (D) 25 and 5 μm, respectively.

Article Snippet: The following antibodies were used for immunofluorescence at the listed concentration: Antibody Species Company Catalog # Concentration CHMP1B Rabbit (polyclonal) Proteintech 14 639-1-AP 1:200 EEA1 Mouse (monoclonal) BD Transduction Laboratories 610 456 1:1000 IST1 Rabbit (polyclonal) Proteintech 19 842-1-AP 1:000 c-myc Mouse (monoclonal) Developmental Studies Hybridoma Bank 9E+10 1:500 FK2 Mouse (monoclonal) Biomol/Enzo PW8810 1:1000 SNX15 Mouse (monoclonal) Santa Cruz sc-393 430 1:200 HRS Mouse (monoclonal) Enzo ALX-804-382 1:1000 VPS35 Rabbit (polyclonal) Thermo Scientific 1:500 Clathrin heavy chain (X22) Mouse (monoclonal) GeneTex GTX22731 1:1000 Cortactin (4F11) Mouse (monoclonal) Millipore 05–180 1:250 WASH Rabbit (polyclonal) Kind gift from Billadeau Lab MC2058 N/A 1:500 AP-1 Mouse (monoclonal) Kind gift from Kornfeld Lab clone 100/3 N/A 1:500 M6PR Rabbit (polyclonal) Kind gift from Kornfeld Lab N/A 1:1000 CHMP4B Rabbit (polyclonal) Kind gift from Shiels Lab N/A 1:200 TfnR Mouse (monoclonal) Santa Cruz sc-32 272 (3B8 2A1) 1:500 Goat anti-rabbit and mouse secondary antibodies conjugated to Alexa 488, 555 or 647 were from Molecular Probes (Thermo Fisher).

Techniques: Staining, Immunostaining, Control, MANN-WHITNEY, Expressing, Live Cell Imaging

FIGURE 2 Tfn retention within the cell is impaired in IST1-depleted cells. (A) T24 cells individually treated with control or IST1 siRNA and then mixed and plated together and stained for TfnR (shown) and IST1 (not shown). (B) T24 cells treated with control or IST1 siRNA were incubated with Tfn Alexa 555 for 1 h, washed with PBS and imaged immediately after adding chase media containing excess unlabeled Tfn. Quantification of mean intensity of Tfn at time 0 min. The graph shows mean ± 95% CI. Control siRNA and IST1 siRNA treated cells n = 110, 120 (n, number pooled from three independent experiments with n = 26, 25, n = 42, 44 and n = 42, 51), respectively. Statistical analysis using student's t test showed a significant decrease in Tfn levels in IST1-depleted cells. (C) Immunoblots of TfnR and IST1 from lysates of cells depleted of IST1 or LAP1 (control) siRNA (D) Quantification of non-permeabilized cells immunolabeled with antibody against the extracellular domain of TfnR to assess TfnR levels on PM. Each point (n = 4 for Control and IST1 siRNA treated cells) represents the average TfnR intensity area for a field of cells (containing >5 cells) normalized by the number of nuclei (i.e., average TfnR intensity per cell). The graph shows median ± 95% CI. Statistical analysis using Mann–Whitney test showed a significant increase in TfnR levels on PM in IST1-depleted cells. This significant increase was replicated in two more independent experiments. (E) Diagram of pulse Tfn assay and quantification of T24 cells allowed to internalize a 30 s pulse of Tfn 555, washed for 1.5 min and then chased with unlabeled Tfn. Cells were fixed at the points indicated, n = 15, 12, 12, 18, 15, 21 and 17 (control) and n = 14, 13, 11, 17, 18, 12 and 12 (IST1 KD). The graph shows median ± 95% CI. Statistical analysis using two-way ANOVA showed Tfn uptake was not impaired in IST1-depleted cells. (F) Fluorescence of Tfn in IST1 siRNA treated and control T24 cells that were fixed (at the indicated times) after internalizing a 30 s pulse of Tfn 555. To compare the localization of Tfn signals, the distribution of an equivalent area (4 μm2) of the highest intensities was assessed by K-means clustering (see Figure S8). The distances of each pixel from the determined cell center were plotted in a histogram. The histograms shown are an average of n = 18, 21 and 17 for control and n = 17, 12 and 12 for IST1siRNA treated cells at 2, 3 and 4 min, respectively. Similar results were observed for three independent experiments. All cell images are max intensity projections. (G) Diagram of the Tfn load-chase assay. (H&I) Time-dependent decay of mean Tfn intensity, measured at 2.5 min intervals after the start of the chase. (H) shows selected time points of 0, 5 and 10 min and (I) is the quantification of all time points, n = 26 for control siRNA and n = 25 IST1 siRNA cells. Error bars are SEM. Inset in I is internal Tfn Intensity normalized for each cell for 0, 2.5 and 5 min timepoints. Control siRNA and IST1 siRNA treated cells n = 110 120 (three independent experiments with n = 26, 25, n = 42, 44 and n = 42,51), respectively. The graph shows mean ± 95% CI. Statistical analysis using two- way ANOVA showed Tfn exocytosis was faster at 2.5 min in IST1-depleted cells. Scale bars in whole cells in (A), (B), (F) and (H) are 25 μm.

Journal: Traffic (Copenhagen, Denmark)

Article Title: IST1 regulates select recycling pathways.

doi: 10.1111/tra.12921

Figure Lengend Snippet: FIGURE 2 Tfn retention within the cell is impaired in IST1-depleted cells. (A) T24 cells individually treated with control or IST1 siRNA and then mixed and plated together and stained for TfnR (shown) and IST1 (not shown). (B) T24 cells treated with control or IST1 siRNA were incubated with Tfn Alexa 555 for 1 h, washed with PBS and imaged immediately after adding chase media containing excess unlabeled Tfn. Quantification of mean intensity of Tfn at time 0 min. The graph shows mean ± 95% CI. Control siRNA and IST1 siRNA treated cells n = 110, 120 (n, number pooled from three independent experiments with n = 26, 25, n = 42, 44 and n = 42, 51), respectively. Statistical analysis using student's t test showed a significant decrease in Tfn levels in IST1-depleted cells. (C) Immunoblots of TfnR and IST1 from lysates of cells depleted of IST1 or LAP1 (control) siRNA (D) Quantification of non-permeabilized cells immunolabeled with antibody against the extracellular domain of TfnR to assess TfnR levels on PM. Each point (n = 4 for Control and IST1 siRNA treated cells) represents the average TfnR intensity area for a field of cells (containing >5 cells) normalized by the number of nuclei (i.e., average TfnR intensity per cell). The graph shows median ± 95% CI. Statistical analysis using Mann–Whitney test showed a significant increase in TfnR levels on PM in IST1-depleted cells. This significant increase was replicated in two more independent experiments. (E) Diagram of pulse Tfn assay and quantification of T24 cells allowed to internalize a 30 s pulse of Tfn 555, washed for 1.5 min and then chased with unlabeled Tfn. Cells were fixed at the points indicated, n = 15, 12, 12, 18, 15, 21 and 17 (control) and n = 14, 13, 11, 17, 18, 12 and 12 (IST1 KD). The graph shows median ± 95% CI. Statistical analysis using two-way ANOVA showed Tfn uptake was not impaired in IST1-depleted cells. (F) Fluorescence of Tfn in IST1 siRNA treated and control T24 cells that were fixed (at the indicated times) after internalizing a 30 s pulse of Tfn 555. To compare the localization of Tfn signals, the distribution of an equivalent area (4 μm2) of the highest intensities was assessed by K-means clustering (see Figure S8). The distances of each pixel from the determined cell center were plotted in a histogram. The histograms shown are an average of n = 18, 21 and 17 for control and n = 17, 12 and 12 for IST1siRNA treated cells at 2, 3 and 4 min, respectively. Similar results were observed for three independent experiments. All cell images are max intensity projections. (G) Diagram of the Tfn load-chase assay. (H&I) Time-dependent decay of mean Tfn intensity, measured at 2.5 min intervals after the start of the chase. (H) shows selected time points of 0, 5 and 10 min and (I) is the quantification of all time points, n = 26 for control siRNA and n = 25 IST1 siRNA cells. Error bars are SEM. Inset in I is internal Tfn Intensity normalized for each cell for 0, 2.5 and 5 min timepoints. Control siRNA and IST1 siRNA treated cells n = 110 120 (three independent experiments with n = 26, 25, n = 42, 44 and n = 42,51), respectively. The graph shows mean ± 95% CI. Statistical analysis using two- way ANOVA showed Tfn exocytosis was faster at 2.5 min in IST1-depleted cells. Scale bars in whole cells in (A), (B), (F) and (H) are 25 μm.

Article Snippet: The following antibodies were used for immunofluorescence at the listed concentration: Antibody Species Company Catalog # Concentration CHMP1B Rabbit (polyclonal) Proteintech 14 639-1-AP 1:200 EEA1 Mouse (monoclonal) BD Transduction Laboratories 610 456 1:1000 IST1 Rabbit (polyclonal) Proteintech 19 842-1-AP 1:000 c-myc Mouse (monoclonal) Developmental Studies Hybridoma Bank 9E+10 1:500 FK2 Mouse (monoclonal) Biomol/Enzo PW8810 1:1000 SNX15 Mouse (monoclonal) Santa Cruz sc-393 430 1:200 HRS Mouse (monoclonal) Enzo ALX-804-382 1:1000 VPS35 Rabbit (polyclonal) Thermo Scientific 1:500 Clathrin heavy chain (X22) Mouse (monoclonal) GeneTex GTX22731 1:1000 Cortactin (4F11) Mouse (monoclonal) Millipore 05–180 1:250 WASH Rabbit (polyclonal) Kind gift from Billadeau Lab MC2058 N/A 1:500 AP-1 Mouse (monoclonal) Kind gift from Kornfeld Lab clone 100/3 N/A 1:500 M6PR Rabbit (polyclonal) Kind gift from Kornfeld Lab N/A 1:1000 CHMP4B Rabbit (polyclonal) Kind gift from Shiels Lab N/A 1:200 TfnR Mouse (monoclonal) Santa Cruz sc-32 272 (3B8 2A1) 1:500 Goat anti-rabbit and mouse secondary antibodies conjugated to Alexa 488, 555 or 647 were from Molecular Probes (Thermo Fisher).

Techniques: Control, Staining, Incubation, Western Blot, Immunolabeling, MANN-WHITNEY, Fluorescence

FIGURE 3 Dispersed Tfn positive compartments in IST1 depleted cells are associated with the endosomal clathrin adaptor AP-1 (A) Control (upper panel) or IST1 depleted T24 cells (lower panel) were treated with 30 s pulse of Tfn555, washed and allowed to continue endocytosis of bound Tfn for 1.5 min before being chased with unlabeled Tfn. Cells were fixed at 30 s (first panel), 1.5 min (second panel), 2.5 min (third panel) or 4 min (fourth panel) and stained for AP-1. (B) Diagram of a short Tfn pulse, where a 30 s pulse of labeled Tfn is immediately followed by chase media and examples of Control KD and IST1 KD cells where IST1 KD cells show AP-1 localization along abnormal peripheral Tfn tubules. The Spearman's Correlation Coefficient between AP-1 and Tfn was quantified for three independent experiments, a total of n = 65 and n = 76 for control and IST1 siRNA-treated cells. The graph shows median and 95% ± CI, and statistical analysis using Mann–Whitney test showed a significant increase in overlap of AP-1 and Tfn in IST1 depleted cells (C) Mixed IST1 siRNA and control T24 cells were fixed before and after treatment with 10 μM brefeldin A (BFA) for 0, 10 and 15 min and stained for TfnR (shown) and IST1 (not shown). All cell images are max intensity projections. Tubules longer than 12 μm within tubular networks were quantified at 0,5,10,15, 30 and 60 min. n ≥10 for each condition, Statistical analysis using two-way ANOVA demonstrated increased tubulation in IST1-depleted cells at 10 and 15 min. Scale bars in whole cells in (A), (B) and (C) are 25 μm, scale bar in insets in (B) is 2.5 μm.

Journal: Traffic (Copenhagen, Denmark)

Article Title: IST1 regulates select recycling pathways.

doi: 10.1111/tra.12921

Figure Lengend Snippet: FIGURE 3 Dispersed Tfn positive compartments in IST1 depleted cells are associated with the endosomal clathrin adaptor AP-1 (A) Control (upper panel) or IST1 depleted T24 cells (lower panel) were treated with 30 s pulse of Tfn555, washed and allowed to continue endocytosis of bound Tfn for 1.5 min before being chased with unlabeled Tfn. Cells were fixed at 30 s (first panel), 1.5 min (second panel), 2.5 min (third panel) or 4 min (fourth panel) and stained for AP-1. (B) Diagram of a short Tfn pulse, where a 30 s pulse of labeled Tfn is immediately followed by chase media and examples of Control KD and IST1 KD cells where IST1 KD cells show AP-1 localization along abnormal peripheral Tfn tubules. The Spearman's Correlation Coefficient between AP-1 and Tfn was quantified for three independent experiments, a total of n = 65 and n = 76 for control and IST1 siRNA-treated cells. The graph shows median and 95% ± CI, and statistical analysis using Mann–Whitney test showed a significant increase in overlap of AP-1 and Tfn in IST1 depleted cells (C) Mixed IST1 siRNA and control T24 cells were fixed before and after treatment with 10 μM brefeldin A (BFA) for 0, 10 and 15 min and stained for TfnR (shown) and IST1 (not shown). All cell images are max intensity projections. Tubules longer than 12 μm within tubular networks were quantified at 0,5,10,15, 30 and 60 min. n ≥10 for each condition, Statistical analysis using two-way ANOVA demonstrated increased tubulation in IST1-depleted cells at 10 and 15 min. Scale bars in whole cells in (A), (B) and (C) are 25 μm, scale bar in insets in (B) is 2.5 μm.

Article Snippet: The following antibodies were used for immunofluorescence at the listed concentration: Antibody Species Company Catalog # Concentration CHMP1B Rabbit (polyclonal) Proteintech 14 639-1-AP 1:200 EEA1 Mouse (monoclonal) BD Transduction Laboratories 610 456 1:1000 IST1 Rabbit (polyclonal) Proteintech 19 842-1-AP 1:000 c-myc Mouse (monoclonal) Developmental Studies Hybridoma Bank 9E+10 1:500 FK2 Mouse (monoclonal) Biomol/Enzo PW8810 1:1000 SNX15 Mouse (monoclonal) Santa Cruz sc-393 430 1:200 HRS Mouse (monoclonal) Enzo ALX-804-382 1:1000 VPS35 Rabbit (polyclonal) Thermo Scientific 1:500 Clathrin heavy chain (X22) Mouse (monoclonal) GeneTex GTX22731 1:1000 Cortactin (4F11) Mouse (monoclonal) Millipore 05–180 1:250 WASH Rabbit (polyclonal) Kind gift from Billadeau Lab MC2058 N/A 1:500 AP-1 Mouse (monoclonal) Kind gift from Kornfeld Lab clone 100/3 N/A 1:500 M6PR Rabbit (polyclonal) Kind gift from Kornfeld Lab N/A 1:1000 CHMP4B Rabbit (polyclonal) Kind gift from Shiels Lab N/A 1:200 TfnR Mouse (monoclonal) Santa Cruz sc-32 272 (3B8 2A1) 1:500 Goat anti-rabbit and mouse secondary antibodies conjugated to Alexa 488, 555 or 647 were from Molecular Probes (Thermo Fisher).

Techniques: Control, Staining, Labeling, MANN-WHITNEY

FIGURE 4 IST1 depletion causes M6PR to accumulate in EEA1-labeled early/sorting endosomes. (A) To compare the localization of M6PR signals, the distribution of an equivalent area (4 μm2) of the highest intensities was assessed by K-means clustering (as shown previously for Tfn in Figure S8). The distances of each pixel from the determined cell center were plotted in a histogram. The histograms shown are an average of n = 25 for control and IST1siRNA-treated cells (data shown is compiled from three independent experiments with each demonstrating increased dispersed M6PR in IST1 siRNA-treated cells). (B) Immunostaining of M6PR and EEA1 in control and IST1-depleted cells. Images are maximum projections of z-stacks obtained on Zeiss LSM 880 with Airyscan and subsequent Airyscan processing. Contrast adjustments on the M6PR channel are equivalent for both conditions, but are different for the EEA1 channel, due to increased EEA1 recruitment in IST1-depleted cells. Scale bars in the whole cells and insets are as follows: (B) 10 and 1 μm, respectively.

Journal: Traffic (Copenhagen, Denmark)

Article Title: IST1 regulates select recycling pathways.

doi: 10.1111/tra.12921

Figure Lengend Snippet: FIGURE 4 IST1 depletion causes M6PR to accumulate in EEA1-labeled early/sorting endosomes. (A) To compare the localization of M6PR signals, the distribution of an equivalent area (4 μm2) of the highest intensities was assessed by K-means clustering (as shown previously for Tfn in Figure S8). The distances of each pixel from the determined cell center were plotted in a histogram. The histograms shown are an average of n = 25 for control and IST1siRNA-treated cells (data shown is compiled from three independent experiments with each demonstrating increased dispersed M6PR in IST1 siRNA-treated cells). (B) Immunostaining of M6PR and EEA1 in control and IST1-depleted cells. Images are maximum projections of z-stacks obtained on Zeiss LSM 880 with Airyscan and subsequent Airyscan processing. Contrast adjustments on the M6PR channel are equivalent for both conditions, but are different for the EEA1 channel, due to increased EEA1 recruitment in IST1-depleted cells. Scale bars in the whole cells and insets are as follows: (B) 10 and 1 μm, respectively.

Article Snippet: The following antibodies were used for immunofluorescence at the listed concentration: Antibody Species Company Catalog # Concentration CHMP1B Rabbit (polyclonal) Proteintech 14 639-1-AP 1:200 EEA1 Mouse (monoclonal) BD Transduction Laboratories 610 456 1:1000 IST1 Rabbit (polyclonal) Proteintech 19 842-1-AP 1:000 c-myc Mouse (monoclonal) Developmental Studies Hybridoma Bank 9E+10 1:500 FK2 Mouse (monoclonal) Biomol/Enzo PW8810 1:1000 SNX15 Mouse (monoclonal) Santa Cruz sc-393 430 1:200 HRS Mouse (monoclonal) Enzo ALX-804-382 1:1000 VPS35 Rabbit (polyclonal) Thermo Scientific 1:500 Clathrin heavy chain (X22) Mouse (monoclonal) GeneTex GTX22731 1:1000 Cortactin (4F11) Mouse (monoclonal) Millipore 05–180 1:250 WASH Rabbit (polyclonal) Kind gift from Billadeau Lab MC2058 N/A 1:500 AP-1 Mouse (monoclonal) Kind gift from Kornfeld Lab clone 100/3 N/A 1:500 M6PR Rabbit (polyclonal) Kind gift from Kornfeld Lab N/A 1:1000 CHMP4B Rabbit (polyclonal) Kind gift from Shiels Lab N/A 1:200 TfnR Mouse (monoclonal) Santa Cruz sc-32 272 (3B8 2A1) 1:500 Goat anti-rabbit and mouse secondary antibodies conjugated to Alexa 488, 555 or 647 were from Molecular Probes (Thermo Fisher).

Techniques: Labeling, Control, Immunostaining

FIGURE 5 IST1 is proximal to early endosome markers HRS, VPS35 and SNX15. IST1 is proximal to recently endocytosed Tfn and known early/sorting endosome domain markers (A) T24 cells allowed to internalize a 30 s pulse of Tfn 555 and were fixed after 2.5 min and stained for IST1 and EEA1. (B) T24 cells stained for IST1 and either HRS, VPS5 or SNX15. All cell images are max intensity projections. Whole cells and insets are as follows: (A) 25 and 1 μm, (B) 25 and 1 μm, respectively.

Journal: Traffic (Copenhagen, Denmark)

Article Title: IST1 regulates select recycling pathways.

doi: 10.1111/tra.12921

Figure Lengend Snippet: FIGURE 5 IST1 is proximal to early endosome markers HRS, VPS35 and SNX15. IST1 is proximal to recently endocytosed Tfn and known early/sorting endosome domain markers (A) T24 cells allowed to internalize a 30 s pulse of Tfn 555 and were fixed after 2.5 min and stained for IST1 and EEA1. (B) T24 cells stained for IST1 and either HRS, VPS5 or SNX15. All cell images are max intensity projections. Whole cells and insets are as follows: (A) 25 and 1 μm, (B) 25 and 1 μm, respectively.

Article Snippet: The following antibodies were used for immunofluorescence at the listed concentration: Antibody Species Company Catalog # Concentration CHMP1B Rabbit (polyclonal) Proteintech 14 639-1-AP 1:200 EEA1 Mouse (monoclonal) BD Transduction Laboratories 610 456 1:1000 IST1 Rabbit (polyclonal) Proteintech 19 842-1-AP 1:000 c-myc Mouse (monoclonal) Developmental Studies Hybridoma Bank 9E+10 1:500 FK2 Mouse (monoclonal) Biomol/Enzo PW8810 1:1000 SNX15 Mouse (monoclonal) Santa Cruz sc-393 430 1:200 HRS Mouse (monoclonal) Enzo ALX-804-382 1:1000 VPS35 Rabbit (polyclonal) Thermo Scientific 1:500 Clathrin heavy chain (X22) Mouse (monoclonal) GeneTex GTX22731 1:1000 Cortactin (4F11) Mouse (monoclonal) Millipore 05–180 1:250 WASH Rabbit (polyclonal) Kind gift from Billadeau Lab MC2058 N/A 1:500 AP-1 Mouse (monoclonal) Kind gift from Kornfeld Lab clone 100/3 N/A 1:500 M6PR Rabbit (polyclonal) Kind gift from Kornfeld Lab N/A 1:1000 CHMP4B Rabbit (polyclonal) Kind gift from Shiels Lab N/A 1:200 TfnR Mouse (monoclonal) Santa Cruz sc-32 272 (3B8 2A1) 1:500 Goat anti-rabbit and mouse secondary antibodies conjugated to Alexa 488, 555 or 647 were from Molecular Probes (Thermo Fisher).

Techniques: Staining

FIGURE 7 IST1 uniquely associates with SNX15. (A) IST1 and SNX15 co-assemble on Tfn-containing endosomes in living U2OS cells. Cell images are single slices. (B) Domain diagram of IST1 (top), SNX15 (bottom) and constructs used to examine the interaction between IST1 and SNX15. (C) Fixed cells expressing SNX15myc with GFP-tagged IST1 constructs shown in B, in the inverted magenta-green images overlapping signal is black and captures the dynamic range of IST1 constructs recruited to SNX15. Cell images are max intensity projections. Scale bars in the whole cells and insets are as follows: (A) 25 and 5 μm, respectively; (C) 25 and 1 μm, respectively.

Journal: Traffic (Copenhagen, Denmark)

Article Title: IST1 regulates select recycling pathways.

doi: 10.1111/tra.12921

Figure Lengend Snippet: FIGURE 7 IST1 uniquely associates with SNX15. (A) IST1 and SNX15 co-assemble on Tfn-containing endosomes in living U2OS cells. Cell images are single slices. (B) Domain diagram of IST1 (top), SNX15 (bottom) and constructs used to examine the interaction between IST1 and SNX15. (C) Fixed cells expressing SNX15myc with GFP-tagged IST1 constructs shown in B, in the inverted magenta-green images overlapping signal is black and captures the dynamic range of IST1 constructs recruited to SNX15. Cell images are max intensity projections. Scale bars in the whole cells and insets are as follows: (A) 25 and 5 μm, respectively; (C) 25 and 1 μm, respectively.

Article Snippet: The following antibodies were used for immunofluorescence at the listed concentration: Antibody Species Company Catalog # Concentration CHMP1B Rabbit (polyclonal) Proteintech 14 639-1-AP 1:200 EEA1 Mouse (monoclonal) BD Transduction Laboratories 610 456 1:1000 IST1 Rabbit (polyclonal) Proteintech 19 842-1-AP 1:000 c-myc Mouse (monoclonal) Developmental Studies Hybridoma Bank 9E+10 1:500 FK2 Mouse (monoclonal) Biomol/Enzo PW8810 1:1000 SNX15 Mouse (monoclonal) Santa Cruz sc-393 430 1:200 HRS Mouse (monoclonal) Enzo ALX-804-382 1:1000 VPS35 Rabbit (polyclonal) Thermo Scientific 1:500 Clathrin heavy chain (X22) Mouse (monoclonal) GeneTex GTX22731 1:1000 Cortactin (4F11) Mouse (monoclonal) Millipore 05–180 1:250 WASH Rabbit (polyclonal) Kind gift from Billadeau Lab MC2058 N/A 1:500 AP-1 Mouse (monoclonal) Kind gift from Kornfeld Lab clone 100/3 N/A 1:500 M6PR Rabbit (polyclonal) Kind gift from Kornfeld Lab N/A 1:1000 CHMP4B Rabbit (polyclonal) Kind gift from Shiels Lab N/A 1:200 TfnR Mouse (monoclonal) Santa Cruz sc-32 272 (3B8 2A1) 1:500 Goat anti-rabbit and mouse secondary antibodies conjugated to Alexa 488, 555 or 647 were from Molecular Probes (Thermo Fisher).

Techniques: Construct, Expressing

FIGURE 8 IST1 assembled with CHMP1B localizes to tubules associated with endosomes, which is distinct from the localization of SNX15. (A–D) SNX15gfp stable cells were fixed either with methanol +BS3 (A, C and D) or with PFA (B) and stained with the indicated antibodies of FAM21/SNX1, WASH/cortactin, CHMP1B/cortactin or IST1/clathrin. All images were deconvolved using Nikon software. Cell images (A–D) are max intensity projections. (E) Quantification of the Pearson's correlation coefficient of SNX15GFP and the indicated marker (left) and manual quantification of the presence of the indicated marker (except for IST1) proximal to SNX15gfp endosomes defined as ROIs (right). (F) Live cell imaging, deconvolved using Nikon software. U2OS cell expressing exogenous SNX15gfp, BFP LifeAct and mcherry cortactin. The panel to the left shows time-lapse imaging of the inset SNX15GFP endosome, as well as line scans that bisect the endosome and the actin domain. Images are single slices. Scale bars in whole cells and insets are as follows: (A–D) 25 and 1 μm (F) 5 and 1 μm, respectively.

Journal: Traffic (Copenhagen, Denmark)

Article Title: IST1 regulates select recycling pathways.

doi: 10.1111/tra.12921

Figure Lengend Snippet: FIGURE 8 IST1 assembled with CHMP1B localizes to tubules associated with endosomes, which is distinct from the localization of SNX15. (A–D) SNX15gfp stable cells were fixed either with methanol +BS3 (A, C and D) or with PFA (B) and stained with the indicated antibodies of FAM21/SNX1, WASH/cortactin, CHMP1B/cortactin or IST1/clathrin. All images were deconvolved using Nikon software. Cell images (A–D) are max intensity projections. (E) Quantification of the Pearson's correlation coefficient of SNX15GFP and the indicated marker (left) and manual quantification of the presence of the indicated marker (except for IST1) proximal to SNX15gfp endosomes defined as ROIs (right). (F) Live cell imaging, deconvolved using Nikon software. U2OS cell expressing exogenous SNX15gfp, BFP LifeAct and mcherry cortactin. The panel to the left shows time-lapse imaging of the inset SNX15GFP endosome, as well as line scans that bisect the endosome and the actin domain. Images are single slices. Scale bars in whole cells and insets are as follows: (A–D) 25 and 1 μm (F) 5 and 1 μm, respectively.

Article Snippet: The following antibodies were used for immunofluorescence at the listed concentration: Antibody Species Company Catalog # Concentration CHMP1B Rabbit (polyclonal) Proteintech 14 639-1-AP 1:200 EEA1 Mouse (monoclonal) BD Transduction Laboratories 610 456 1:1000 IST1 Rabbit (polyclonal) Proteintech 19 842-1-AP 1:000 c-myc Mouse (monoclonal) Developmental Studies Hybridoma Bank 9E+10 1:500 FK2 Mouse (monoclonal) Biomol/Enzo PW8810 1:1000 SNX15 Mouse (monoclonal) Santa Cruz sc-393 430 1:200 HRS Mouse (monoclonal) Enzo ALX-804-382 1:1000 VPS35 Rabbit (polyclonal) Thermo Scientific 1:500 Clathrin heavy chain (X22) Mouse (monoclonal) GeneTex GTX22731 1:1000 Cortactin (4F11) Mouse (monoclonal) Millipore 05–180 1:250 WASH Rabbit (polyclonal) Kind gift from Billadeau Lab MC2058 N/A 1:500 AP-1 Mouse (monoclonal) Kind gift from Kornfeld Lab clone 100/3 N/A 1:500 M6PR Rabbit (polyclonal) Kind gift from Kornfeld Lab N/A 1:1000 CHMP4B Rabbit (polyclonal) Kind gift from Shiels Lab N/A 1:200 TfnR Mouse (monoclonal) Santa Cruz sc-32 272 (3B8 2A1) 1:500 Goat anti-rabbit and mouse secondary antibodies conjugated to Alexa 488, 555 or 647 were from Molecular Probes (Thermo Fisher).

Techniques: Staining, Software, Marker, Live Cell Imaging, Expressing, Imaging